Yep, I’m not pushing the peristaltic pump idea as a necessity. Works for me but not the only solution. Ultimately, you need something mechanical to push water through. Anything mechanical can fail peristaltic or otherwise. You have to find what works for you.
I reviewed 204 & 205 and agree. If it ain’t broke, don’t fix it.
If you do decide to stick with the peri-pump... Even if it’s in a push configuration, 5ml in should guarantee 5ml out. The way they’re built they won’t allow water flow either direction when the pump is turned off. It pinches the tube as a part of the peristaltic action. That squirts out metered amounts through the output. When the pump is turned off, there is no way for air to enter the system.
Imagine filling a straw with liquid in a cup and pinching the top. Pull the straw out of the liquid and a majority remains inside the straw until you take your finger off the top. That’s what happens in a properly sealed reactor with the peristaltic pump. It’s pinching small amounts of liquid and pushing it forward. Then the next roller comes along when the head rotates and pinches another small amount moving it forward.
Peristalsis is a medical term that describes, for example, how we move food through our bodies. We subconsciously contract sequential muscles internally which moves food along the digestive track towards the inevitable.
Still, you have to go with what works. Maybe a squirrel on a small bike powering a turbine?