I have a tendency to overbuild things.
If I'm doing the math right, theres's no way you'll exceed 500 lbs for that tank assuming a full 50 gal tank, which you'll be under by at least 6-10 gal depending on depth.
I would feel comfortable with that on 2x4's and plywood. Jodil brings up a valid point, fill it up and see what the deflection is. I doubt you'll see any. I would bet that 2x4's would be enough on their own. If you have any doubt, you could add some glue or liquid nails between the lumber and ply to solidify the structure (and some screws to hold everything together). That would lock all of the lumber together and help to distribute the load.
In my experience, those tanks are pretty strong to begin with, so I think you'll be safe.